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Selecting the proper antibody for your research project is a crucial step that can make or break your experience at the bench. While there is no shortage of options in the market, taking a moment to carefully consider your research goals before making your purchase can save you time and money down the road.

One antibody that works for multiple experiments may not be the best choice for all applications. Therefore, it is important to review your options each time you start a new research plan. To know more about antibodies, you can also contact the best antibody production companies via https://www.bosterbio.com/services/custom-antibody-production-services.

While most experiments require at least some adjustment, there are some important questions to ask yourself before buying a new antibody:

Antibody

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What is my sample source?

Perhaps the most basic consideration, the source of the sample you wish to test – human, mouse, goat, mouse or otherwise – determines which antibody will bind to the desired molecule. Antibodies must be produced in a different form from the sample source (for example, goat antibodies against human tissue samples may be used).

What application do I intend for this antibody?

While the desired protein in your sample may remain the same, the experiment you want to perform may change, potentially requiring changes to your antibodies. Consider sample preparation carefully: Western blot and IP protocols require proteins to be denatured, indicating antibody-binding sites that may not be exposed during ELISA, IHC, or FACS.

Is the antibody validated?

Before investing time and money in a pilot trial or full-scale study, it is recommended that your protocol be validated externally.

Many company websites offer pre-validation and results of their most popular antibody tests. However, given the number of products, it is common to offer antibodies for sale without prior validation.

Questions To Ask Before Purchasing An Antibody